Cytotoxic Activity of Gallic Acid and Myricetin against Ovarian Cancer Cells by Production of Reactive Oxygen Species

Some studies demonstrate that gallic acid (GA) and myricetin (MYR) isolated from Rhus trilobata provide the therapeutic activity of this plant against cancer. However, few reports both compounds could also have potential in ovarian Therefore, evaluating cytotoxic GA MYR cancer cells determining possible action mechanism present are important. For purpose, SKOV-3 (ovarian adenocarcinoma; HTB-77™, ATCC®) were cultivated according to supplier’s instructions (37 °C 5% CO2) determine biological by confocal/transmission electron microscopy, PI-flow cytometry, H2DCF-DA, MTT, Annexin-V assays. Possible molecular targets determined Similarity Ensemble approach. Results showed treatments decreased viability at 50 166 μg/mL, respectively (p ≤ 0.05, ANOVA vs. vehicle group). They induced morphological changes (cytoplasmic reduction, nuclear chromatin condensation, cytoplasmic vesicles increment, polymerized actin, stabilized tubulin), cell cycle arrest (GA: 8.3% G2/M MYR: 78% G1), apoptosis induction 18.9% 8.1%), due ROS generation (34 42%) for 24 h In silico demonstrated interact with carbonic anhydrase-IX PI3K, respectively. conclusion, show through production, which modifies cytoskeleton induces apoptosis. be considered as base development new chemotherapy